Review




Structured Review

Merck & Co pet14b
Pet14b, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet14b/pet14b/pm39282643-277-25-27
Average 90 stars, based on 1 article reviews
pet14b - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Virus:

Article Title: Different molecular recognition by three domains of the full-length GRB2 to SOS1 proline-rich motifs and EGFR phosphorylated sites.
Article Snippet: .. The gene encoding human GRB2 in addition to an N-terminal His6 tag with a tobacco etch virus (TEV) protease cleavage site was constructed into the vector pET14b (Merck) for overexpression in the Escherichia coli BL21 (DE3) pLysS strain. .. Uniformly 13C/15N-labelled GRB2 protein was obtained by growing the bacteria at 37 °C in M9 minimal media, containing [13C6]-glucose (ISOTEC) and 15NH4Cl (ISOTEC) as the sole carbon and nitrogen sources, respectively, supplemented with 20 mM MgSO4, 0.1 mM CaCl2, 0.4 mg mL −1 thiamin, 20 mM FeCl3, salt mix (4 mM ZnSO4, 0.7 mM CuSO4, 1 mM MnSO4, 4.7 mM H3BO3), and 50 mg L −1 ampicillin.

Article Title: Different molecular recognition by three domains of the full-length GRB2 to SOS1 proline-rich motifs and EGFR phosphorylated sites
Article Snippet: .. The gene encoding human GRB2 in addition to a N-terminal His 6 tag with a tobacco etch virus (TEV) protease cleavage site was constructed into the vector pET14b (Merck) for over-expression in the Escherichia coli BL21 (DE3) pLysS strain. .. Uniformly 13 C/ 15 N-labelled GRB2 protein was obtained by growing the bacteria at 37 °C in M9 minimal media, containing [ 13 C 6 ]-glucose (ISOTEC) and 15 NH 4 Cl (ISOTEC) as the sole carbon and nitrogen sources, respectively, supplemented with 20 mM MgSO 4 , 0.1 mM CaCl 2 , 0.4 mg/ml thiamin, 20 μM FeCl 3 , salt mix (4 μM ZnSO 4 , 0.7 μM CuSO 4 , 1 μM MnSO 4 , 4.7 μM H 3 BO 3 ), and 50 mg/l ampicillin.

Construct:

Article Title: Different molecular recognition by three domains of the full-length GRB2 to SOS1 proline-rich motifs and EGFR phosphorylated sites.
Article Snippet: .. The gene encoding human GRB2 in addition to an N-terminal His6 tag with a tobacco etch virus (TEV) protease cleavage site was constructed into the vector pET14b (Merck) for overexpression in the Escherichia coli BL21 (DE3) pLysS strain. .. Uniformly 13C/15N-labelled GRB2 protein was obtained by growing the bacteria at 37 °C in M9 minimal media, containing [13C6]-glucose (ISOTEC) and 15NH4Cl (ISOTEC) as the sole carbon and nitrogen sources, respectively, supplemented with 20 mM MgSO4, 0.1 mM CaCl2, 0.4 mg mL −1 thiamin, 20 mM FeCl3, salt mix (4 mM ZnSO4, 0.7 mM CuSO4, 1 mM MnSO4, 4.7 mM H3BO3), and 50 mg L −1 ampicillin.

Article Title: Different molecular recognition by three domains of the full-length GRB2 to SOS1 proline-rich motifs and EGFR phosphorylated sites
Article Snippet: .. The gene encoding human GRB2 in addition to a N-terminal His 6 tag with a tobacco etch virus (TEV) protease cleavage site was constructed into the vector pET14b (Merck) for over-expression in the Escherichia coli BL21 (DE3) pLysS strain. .. Uniformly 13 C/ 15 N-labelled GRB2 protein was obtained by growing the bacteria at 37 °C in M9 minimal media, containing [ 13 C 6 ]-glucose (ISOTEC) and 15 NH 4 Cl (ISOTEC) as the sole carbon and nitrogen sources, respectively, supplemented with 20 mM MgSO 4 , 0.1 mM CaCl 2 , 0.4 mg/ml thiamin, 20 μM FeCl 3 , salt mix (4 μM ZnSO 4 , 0.7 μM CuSO 4 , 1 μM MnSO 4 , 4.7 μM H 3 BO 3 ), and 50 mg/l ampicillin.

Over Expression:

Article Title: Different molecular recognition by three domains of the full-length GRB2 to SOS1 proline-rich motifs and EGFR phosphorylated sites.
Article Snippet: .. The gene encoding human GRB2 in addition to an N-terminal His6 tag with a tobacco etch virus (TEV) protease cleavage site was constructed into the vector pET14b (Merck) for overexpression in the Escherichia coli BL21 (DE3) pLysS strain. .. Uniformly 13C/15N-labelled GRB2 protein was obtained by growing the bacteria at 37 °C in M9 minimal media, containing [13C6]-glucose (ISOTEC) and 15NH4Cl (ISOTEC) as the sole carbon and nitrogen sources, respectively, supplemented with 20 mM MgSO4, 0.1 mM CaCl2, 0.4 mg mL −1 thiamin, 20 mM FeCl3, salt mix (4 mM ZnSO4, 0.7 mM CuSO4, 1 mM MnSO4, 4.7 mM H3BO3), and 50 mg L −1 ampicillin.



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